Table 2.
IL-β and TNF-α secretion induced by calcitriol and its analogue in breast cancer cell lines.
| SUM-229PE | HCC1806 | MCF7 | |||||
|---|---|---|---|---|---|---|---|
| IL-1β (pg/ml) | TNF-α (pg/ml) | IL-1β (pg/ml) | TNF-α (pg/ml) | IL-1β (pg/ml) | TNF-α (pg/ml) | ||
| Calcitriol (nM) | 0 | 14.9 ± 8.9 | 7.1 ± 2.2 | 29.2 ± 2.5 | 28.4 ± 9.9 | 15.4 ± 4.9 | 27.9 ± 10.5 |
| 1 | 16.3 ± 1.2 | 10.5 ± 1.7 | 24.9 ± 1.7 | 23.7 ± 0.4 | 13.8 ± 4.0 | 34.3 ± 9.9 | |
| 10 | 19.1 ± 2.3 | 22.9 ± 6.6∗ | 31.3 ± 1.8 | 19.9 ± 1.7 | 12.8 ± 2.4 | 26.7 ± 3.1 | |
| 100 | 33.4 ± 2.4∗ | 10.9 ± 3.1 | 32.1 ± 3.4 | 20.3 ± 1.0 | 20.5 ± 3.4 | 36.4 ± 8.2 | |
|
| |||||||
| EB1089 (nM) | 1 | 39.2 ± 11.2∗ | 13.1 ± 1.3∗ | 46.4 ± 3.4∗ | 23.5 ± 6.3 | 11.76 ± 4.5 | 24.5 ± 8.6 |
| 10 | 24.3 ± 0.9∗ | 17.9 ± 0.9∗ | 41.7 ± 3.4∗ | 21.3 ± 6.2 | 13.54 ± 1.3 | 25.0 ± 11.4 | |
| 100 | 27.4 ± 5.1∗ | 17.7 ± 1.6∗ | 38.7 ± 0.8∗ | 28.4 ± 7.3 | 17.84 ± 6.6 | 35.7 ± 0.7 | |
Results are expressed as the mean ± SD cytokine secretion of triplicate determinations and represent at least three different experiments. ∗ P < 0.05 vs. nontreated cells (0).