Table 1.

Components of standard cell-free protein synthesis (CFPS) system.

Components	Volume (μL)
10 × salt	2
PEP	1.6
Mg2+	0.4
Extract	5
T7 polymerase	0.2
NTPs mixture	0.8
Amino acids mixture	0.8
Plasmid template	1 (300 ng)
o-tDNA	1 (100 ng/μL)
pPaFRS	1 (5 mM)
ddH2O	6.12
Total	20

PEP: Phosphoenolpyruvic acid; pPaFRS: p-propargyloxyphenylalanine.