Table 1.
Components | Volume (μL) |
---|---|
10 × salt | 2 |
PEP | 1.6 |
Mg2+ | 0.4 |
Extract | 5 |
T7 polymerase | 0.2 |
NTPs mixture | 0.8 |
Amino acids mixture | 0.8 |
Plasmid template | 1 (300 ng) |
o-tDNA | 1 (100 ng/μL) |
pPaFRS | 1 (5 mM) |
ddH2O | 6.12 |
Total | 20 |
PEP: Phosphoenolpyruvic acid; pPaFRS: p-propargyloxyphenylalanine.