Skip to main content
. 2019 Apr 9;2019:4982062. doi: 10.1155/2019/4982062

Figure 1.

Figure 1

Inhibition of JAK3 phosphorylation activity (a–c). Inhibition of JAK3 (%) by 3 Chinese herbal compounds: (a) Cryptotanshinone (CRY), (b) Icaritin (ICA), and (c) Indirubin (IND), at various concentrations. JAK3 phosphorylation was measured using monoclonal antiphosphotyrosine–peroxidase conjugated antibody. Data represent means ± SEM of three independent experiments. The self-simulation (STIM) without inhibitors indicated the maximum phosphorylation. Compound CP690,550 was selected as positive control. Inhibition of cellular JAK3-mediated STAT5 activity (d–f). Inhibitory effect of treatment with 3 Chinese herbal compounds on luciferase activity of STAT5 at various concentrations. HEK 293 cells were transfected with a STAT5-dependent luciferase reporter then treated with herbal compounds for 1 h prior to conducting the luciferase reporter assay. Data represent means ± SEM of three independent experiments. ∗∗∗p <0.001 versus control group; significance was determined using one-way ANOVA.