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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: DNA Repair (Amst). 2019 Feb 16;76:30–39. doi: 10.1016/j.dnarep.2019.02.005

Figure 4. Chemical reaction mechanism of catalysis by USP7.

Figure 4.

The catalytic triad of USP7 is shown on the left. Cleavage of ubiquitinated substrate is shown on the right. The substrate and ubiquitin are shown in pink and green, respectively and labeled. The nucleophilic attack of the isopeptide bond is carried out by C223 of the catalytic triad. H464 serves as a proton acceptor and D481 restricts the side-chain rotation of the histidine.