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. Author manuscript; available in PMC: 2019 Apr 24.
Published in final edited form as: Chem Res Toxicol. 2018 Jun 26;31(7):548–555. doi: 10.1021/acs.chemrestox.8b00023

Figure 2.

Figure 2.

Roles of CYPs in bioactivation of ILB (formation of M2) and M1 (formation of M19 and M20). (A) The formation of M2 is NADPH dependent in the incubation with human liver microsomes (HLM). N.D. not detected. (B) Role of CYPs in M2 formation. The relative abundance of M2 from the incubation with CYP3A4 was set as 100%. (C) Effect of KCZ and fluoxetine on M2 formation in the incubation with HLM. KCZ and fluoxetine were used as an inhibitor of CYP3A4 and CYP2C9, respectively. (D) Effect of KCZ and fluoxetine on M2 formation in the incubation with cDNA-expressed CYP3A4 and CYP2C9, respectively. The relative abundance of M2 in control groups was set as 100%. (E) Role of CYPs in M19 formation. The relative abundance of M19 from the incubation with CYP2C9 was set as 100%. (F) Role of CYPs in M20 formation. The relative abundance of M20 from the incubation with CYP3A4 was set as 100%. All the data are expressed as means ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.