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(A) aSMase expression and activity (B) was suppressed significantly by transfecting aSMase siRNA. Elevated aSMase activity (C) and ceramide content (D) induced by HG was suppressed by desipramine treatment and transfecting aSMase siRNA. Data are shown as the mean ± S.E.M. with n=4–5 per group. *P<0.05 compared with the NC group. #P<0.05 compared with the HG group.
