Table 1.

Primers used in this study.

Species	Primer	Sequence (5′-3′)	References	Concentration(nm)	Annealing conditions
G. vaginalis	GVF	TTACTGGTGTATCACTGTAAGG	De Backer et al., 2007	200	55°C−45 s
	VR	CCGTCACAGGCTGAACAGT		200
A.vaginae	AVF	CCCTATCCGCTCCTGATACC	Menard et al., 2008	200	60°C−60 s
	AVR	CAAATATCTGCGCATTTCA		200
S. agalactiae	SagalactF	TTCACCAGCTGTATTAGAAGTACATGC	Van den Brand et al., 2014	500	60°C−60 s
	SagalactR	CCCTGAACATTATCTTTGATATTTCTCA		500
P. acnes	PAF	GCGTGAGTGACGGTAATGGGTA	Miura et al., 2010	100	60°C−60 s
	PAR	TTCCGACGCGATCAACCA		100
	PAP	FAM-AGCGTTGTCCGGATTTATTGGGCG-TAMRA		100