Table 7.
Quantification of organic acids after infection assays (Mean ± SD).
Organic acids concentration (g/L) | ||||
---|---|---|---|---|
Acetic acid | Lactic acid | Propionic acid | Succinic acid | |
Control (Both + Epitelium) | 1.314 ± 0.062 | 0.399 ± 0.386 | <LOQ | 0.126 ± 0.002 |
Control A. vaginae | 1.117 ± 0.111 | 0.420 ± 0.525 | <LOQ | 0.050 ± 0.001 |
Control G. vaginalis | 1.043 ± 0.450 | 0.516 ± 0.410 | <LOQ | 0.051 ± 0.001 |
Control P. acnes | 1.203 ± 0.635 | 0.616 ± 0.497 | 0.829 ± 0.869a* | 0.073 ± 0.036 |
Control St. agalactiae | 1.217 ± 0.246 | 5.172 ± 0.314a** | <LOQ | 0.063 ± 0.019 |
Control BPL005 | 1.371 ± 0.122 | 4.769 ± 0.145a** | 0.093 ± 0.002a* | 0.045 ± 0.001 |
BPL005 + A. vaginae | 1.325 ± 0.103 | 4.515 ± 0.403b** | 0.139 ± 0.005b* | 0.048 ± 0.001 |
BPL005 + G. vaginalis | 1.338 ± 0.073 | 4.392 ± 0.223b** | 0.142 ± 0.010b* | 0.046 ± 0.003 |
BPL005 + P. acnes | 1.337 ± 0.206 | 4.160 ± 1.189b** | 0.309 ± 0.011 | 0.067 ± 0.024 |
BPL005 + St. agalactiae | 1.264 ± 0.021 | 4.800 ± 0.002 | <LOQ | 0.048 ± 0.002 |
LOQ, Limit of quantification = 0.01 g/L (lactic acid, succinic acid, propionic acid, butyric acid) and 0.05 g/L (acetic acid). Statistics are done:
comparing control (broth + epithelium) vs. control of pathogen, are statistically different;
each pathogen control vs. its grown together with strain BPL005, are statistically different.
p-value < 0.05
p-value < 0.001.