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. 2019 Mar 15;10(22):2136–2150. doi: 10.18632/oncotarget.26669

Figure 3. Quantitative pSILAC data reveals primary hypoxia responsible proteins in cancer cells.

Figure 3

(A) Hypoxia induced protein synthesis of oxidative stress markers and ER stress response proteins in A431 cells grown in serum free medium. Induction fold was normalized by pre-existing proteins. Error bars represent the mean±s.d., n=2. **p<0.01, *p<0.05. (B) Serum depletion induced protein synthesis of angiogenesis related proteases and cancer biomarkers in A431 cells. Induction fold was normalized by pre-existing proteins. Error bars represent the mean±s.d., n=2. *p<0.05. (C–D) qRT-PCR analysis of 5 selected genes in A431, HCT116, and SW480 cells showed increase of mRNA expression under hypoxia. Error bars represent the mean ± s.d., n=3. ***p<0.001. Abbreviations: Nx, normoxia; Hx, hypoxia; SF, serum free.