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. 2019 Mar 19;10(23):2320–2334. doi: 10.18632/oncotarget.26798

Figure 2. Copy number of MET gene in thyroid cancer cell lines.

Figure 2

(A) Fluorescence in situ hybridization in 4 thyroid cancer cell lines. MET probe: green; centromeric probe for chromosome 7 (CEP7): red; nuclei: blue (DAPI) (magnification ×60). (B) Relative copy number of MET gene in 11 human thyroid cancer cell lines. MET copy number was estimated by relative quantification of the MET gene locus with an unrelated control locus, COL6A5 at chromosome locus 3q22.1 by SYBR®Green qPCR. Calibrator DNA was leukocytes DNA from a healthy subject and control DNA was extracted from another healthy subject. A copy number >4 has been considered significant. The HEPG2 liver cancer cell line served as control.