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. 2019 Mar 19;10(23):2320–2334. doi: 10.18632/oncotarget.26798

Figure 6. MET activation is required for cell migration and invasion in TTA1 cells.

Figure 6

(A) TTA1 cells were treated with PHA665752 200 nM or not for 24 hours and plated in triplicate in a soft agar medium. Low magnification image capture of one representative soft agar culture of control cells and PHA665752-treated cells are reported. (B) Two days after transfection with the MET siRNA1 and MET si-RNA2, TTA1 cells were plated in soft agar medium in triplicate. Colonies were counted on day 12. The mean results of triplicate of one representative experiment are reported. (C) Cell migration and invasion of TTA1 cells treated with the indicated doses of PHA665752 over a period of 24 h were assessed in transwell assays. The mean results of three independent migration and invasion experiments performed in duplicate are reported (*p < 0.05; **p < 0.01). (D) Western blot analysis of activated and total MET, ERK1/2, AKT in TTA1 cells subjected to migration and invasion assays. (E) Migration and invasion of TTA1 cells transfected with MET si-RNA1 and MET si-RNA2 (right panel), compared to cell migration and invasion of PHA665752-treated cells (left panel). The mean results of two independent migration and invasion experiments carried out in duplicate are reported (*p < 0.05; **p < 0.01)”.