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. 2019 Mar 19;10(23):2237–2251. doi: 10.18632/oncotarget.26787

Figure 4. SAB298 suppresses SFK and PI3K activities.

Figure 4

Western blot analyses of melanoma cells treated with DMSO or SAB298 (0.5 µM) for 5 hrs. Cell extracts were probed with antibodies to phosphorylated SFKs (SFK pY416), phosphorylated PI3KR1/3 (pY467/199), to different SFKs or PIK3R as indicated. Reduction in the levels of pSFK Y416 in response to SAB298 indicates inhibition of SFK. Two cell lines (SK-MEL-28 and YUSEEP) did not express detectable levels of SFK pY416. Anti-actin represents protein loading. The levels of growth responses to SAB298 are indicated by the IC50, AUC1 and AUC2 on the bottom of each lane. YUSIV and YUSEEP carry PDE8A-RAF1 or GOLGA4-RAF1 fusion proteins, respectively.