Rat embryo E14. Frontal section of the floor of the mouth. (a) Haematoxylin‐eosin staining. The medial paralingual groove is beginning to form (arrow). Primordium of the PSG (framed by a dashed line) in relationship to the lingual nerve (L). T, tongue; V, vessel. (b) HNK‐1 immunoperoxidase labelling. HNK‐1 is positive to the lingual nerve (L), the inferior alveolar nerve (I) and the mesenchyme related to the lingual nerve (arrowheads). Arrow, medial paralingual groove. (c) Magnification of (a). HNK‐1 is positive to the lingual nerve (L), the inferior alveolar nerve (I) and the mesenchyme related to the lingual nerve, which corresponds to the primordium of the parasympathetic submandibular ganglion (arrowheads).