Fig. 2.

Tethering of donor and acceptor liposomes in the absence or presence of E-Syt1_cyto (protein: lipid ratio 1:2000) and 100 μM Ca²⁺, as assessed by increase in turbidity (OD at 405 nm). A cocktail of EGTA, imidazole, and Proteinase K (Cocktail) was added after 10 min. Time-courses are at left and bar graphs showing quantification of OD₄₀₅ increases at the time of addition of the cocktail (arrows in the left panel) are at right. Mean and SD of three independent experiments