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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Cell Immunol. 2019 Mar 19;338:32–42. doi: 10.1016/j.cellimm.2019.03.003

Figure 5. Detection of cytokines secreted by dendritic cells treated with CTB-INS.

Figure 5.

Panel A, Graphic representation of ELISA assay of DC secretion of the pro-inflammatory cytokine IL-12p70 detected in RPMI culture medium after 24 h treatment of moDCs with PBS (NC), 1μg/mL of LPS, 10 μg/mL of CTB-INS containing residual LPS, 10 μg/mL of CTB-INS treated with Triton X-114 and Endotrap resin to remove residual LPS. Panel B is a graphic representation of DC secretion of the anti-inflammatory cytokine IL-10. The error bars represent the standard deviation from the mean of three independent ELISA experiments. *p < 0.05, in comparison with the NC.