Figure 5.

Increased inulin provision abolishes effects of PCB 126 treatment on fermentative SCFA production by isolated fecal bacterial. Bacterial cell suspensions were maintained on inulin substrate (10g/L) and subsequently exposed to PCB 126 (0.02 μM, 0.2 μM, 2 μM) or vehicle control (DMSO) for 48h (n=3). HPLC was utilized to quantify fermentation acids in the media after incubation. PCB 126 had no significant effect on fermentation acid production from a higher inulin substrate concentration (10 g/L). There was a trend towards lower total fermentation acid production (p=0.055) in bacterial cultures exposed to higher levels of PCB 126. All data are presented as means ± SEM. * Significantly different compared to vehicle control (DMSO) (p<0.05; Student’s t-test)