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. 2018 Jun 19;2(2):026111. doi: 10.1063/1.5025216

FIG. 6.

FIG. 6.

Junctional proteins recruitment upon mechanical stimulation. (a) Representative and averaged images of E-cadherin (n = 20 junctions), ZO-1 (n = 8 junctions), and occludin (n = 11 junctions) images for a 10-min 1-sided stimulation. Paired imaged (noStr and Str) were normalized from 0 to 1. (b) Punctate analysis (ΔI: relative recruitment in %) of E-cadherin (n = 250–450 puncta), ZO-1 (n = 100–250 puncta), and occludin (n = 150–250 puncta) upon 10-min 1-sided stimulation. Only the stretched junction side showed reinforcement (E-cadherin by 34.3 ± 4.0%s.e.m., ZO-1 by 14.6 ± 3.9%s.e.m., and occludin by 28.5 ± 3.4%s.e.m. Adjusted for photo-bleaching. Such an effect is however abolished in the presence of Jasplakinolide. Com: Compressed side; Str: stretched side; and noStr: no stimulation case. Statistics has been performed via the two-sample t-test with * for p < 0.05, ** for p < 1 × 10−2, *** for p < 1 × 10−3, **** for p < 1 × 10−4, and ***** for p < 1 × 10−5. Mean, 25th, and 75th percentiles are indicated as the boxed bar.