FIG. 7.
Junction protein mechanosensitivities at or in-between E-cadherin puncta. Intensities of E-cadherin, actin, ZO-1, and occludin under cadherin puncta (P) (n = 60–200 puncta) or in the space of the in-between puncta region (IP) (n = 50–100 regions) in the stretched (10 min stimulation) and non-stretched cases. Note i—the general reduction of all protein levels at regions in between puncta, compared to puncta region; ii—the absence of stretch-induced recruitment of E-cadherin at its in-between puncta regions; iii—the persistence of stretch-induced recruitment of the other proteins at in-between E-cadherin puncta regions. noStr: no stimulation case; Str: stretched case. Statistics has been performed via the two-sample t-test with * for p < 0.05, ** for p < 1 × 10−2, *** for p < 1 × 10−3, **** for p < 1 × 10−4, and ***** for p < 1 × 10−5. Mean, 25th, and 75th percentiles are indicated on the bar.