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. 2018 Dec 26;2(4):046107. doi: 10.1063/1.5052239

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© 2018 Author(s).

2473-2877/2018/2(4)/046107/16

All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FIG. 1. — Microcontact-printed ECM screening arrays. (a) Photomask containing 252 individual ECM conditions, each containing a unique randomly generated array of line elements with varying alignments (angular dispersion), line densities, and line widths. (b) Overview of the negative microcontact-printing procedure. Briefly, AlexaFluor555-conjugated fibronectin (Fn555, red) was uniformly coated on flat PDMS stamps and a UV-ozone activated stamp containing the negative of the intended patterns was applied to remove background areas before transferring the remaining areas to the eventual cell culture substrate. (c) Tile-scan fluorescence image of a subsection of the Fn-printed (red) ECM screening array seeded with phalloidin-AlexaFluor488 stained 3T3s (green) (scale bar: 1 mm). (d) Fn555 microcontact-printed patterns demonstrating control over line alignment (left), line density (middle), and line width (right) (scale bars: 10 μm).