Fig 7. Pannexin1 localization is detected at wound edge during healing.

(A) Representative confocal immunofluorescence images of cultured cells stained for pannexin1 localization (yellow, including arrowheads) and counterstained with rhodamine phalloidin (red). Pannexin1 is concentrated adjacent to the leading edge of the wound. Scale bar = 23 μm. (n = 3). (B) Representative confocal immunofluorescence images of basal corneal epithelium stained for pannexin1 (yellow, including arrows) and counterstained for rhodamine phalloidin (red). After wounding (2 and 4 hours), the pannexin1 concentrated towards the leading edge of the wound. An asterisk indicates the leading edge of migrating epithelium. Scale bar = 18.5 μm. (n = 3). (C) Ca²⁺ mobilizations are represented over time in event charts of LE and BFLE cells 10 minutes after wounding, with cells preincubated with inhibitory peptide 10Panx or scrambled peptide control (Ctrl) (n = 3). (D) Event probability values for LE cells after wounding for both treated and Ctrl groups. Data are mean ± SEM and were analyzed with a one-way ANOVA with the Tukey’s multiple comparisons test (**p<0.01, n = 3).