Fig 4. Fatty acids effects on osteogenic genes expression.

Relative expression of genes related to the process of osteogenesis (fib1a, mgp, on and op) normalized to b-actin and rps18 in gilthead sea bream bone-derived cells. Cells at day 4 were treated with different (A) individual or (B) combined fatty acids or were left untreated as control (dashed lines) for 6 h. Data are shown as mean + SEM (n = 3–4). Significant differences (p<0.05) among treatments are indicated by different letters. Asterisks indicate significant differences (p<0.05) with the control. EPA: eicosapentaenoic acid; DHA: docosahexaenoic acid; LA: linoleic acid; ALA: α-linolenic acid.