Figure 5:

Representative sedimentation equilibrium analytical ultracentrifugation data. This sample was centrifuged at 15000 rpm in 50 mM Na⁺/K⁺ phosphate, 0.1% sodium azide, and 200 mM NaCl, pH 7.0. Equilibrium was achieved after approximately 12 h at 20 °C. The residuals determined based on nonlinear least-squares curve fitting are shown on the upper panel. All of the samples were fit in a similar manner to a single sedimenting species. The molecular weight for this sample was determined to be 72953 Da.