Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene | TK0986 | UniProt database | Q5JID0 | |
| Strain, strain background (E. coli) | MC1061 | Casadaban and Cohen, 1980 | ||
| Biological sample (Thermococcus kodakarensis KOD1) |
ATCC BAA-918/JCM 12380/KOD1 | |||
| Recombinant DNA reagent | pBAD24-GltTk-His8 | Jensen et al., 2013 | Expression plasmid for C-terminally His8-tagged GltTk. | |
| Chemical compound | D-Asp | Sigma-Aldrich | 219096–25G | ReagentPlus99% |
| Software | Origin 8 | OriginLab | ||
| Other | GltTk-D-aspartate coordinate file and structural factors | This paper | accession number PDB ID code 6R7R |
Crystal structure of the glutamate transporter homologue GltTk in complex with D-aspartate |