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. 2019 Apr 24;5(4):eaaw3754. doi: 10.1126/sciadv.aaw3754

Fig. 1. Three S. pennellii LA0716 regions condition acylglucose accumulation.

Fig. 1

(A) Examples of nuclear magnetic resonance (NMR)–resolved S. lycopersicum and S. pennellii acylsugar structures. Acylsugars from S. lycopersicum are composed of sucrose acylated on both the pyranose and furanose rings (“F-type”). S. pennellii acylsugars are a mixture of sucrose-based (“P-type”) and glucose-based compounds with acylation exclusively on the pyranose ring. (B) Acylsugar electrospray ionization (ESI−)–mode LC-QToF (Quadrupole time-of-flight) MS profiles. Top: S. lycopersicum M82 with acylsucroses S3:15 (5,5,5)-F, S4:17 (2,5,5,5)-F, S3:22 (5,5,12)-F, and S4:24 (2,5,5,12)-F annotated. Bottom: S. pennellii LA0716 acylsucroses and acylglucoses. (C) Left: Representation of S. pennellii chromosomal introgressions in BIL6521 × BIL6180 progeny that contain quantitative trait loci (QTLs) affecting acylglucose biosynthesis (30). The black portions of the chromosomes correspond to S. pennellii introgressions, while the white portions correspond to the chromosomal regions in the M82 background. Right: ESI− mode liquid chromatography–mass spectrometry (LC-MS) analysis of BIL6180 compared with the BIL6180 × BIL6521 F2 progeny reveals acylglucose accumulation in the hybrid, but not in BIL6180. All ESI− mode acylsugars were identified as formate adducts. Chr., chromosome.