Skip to main content
. 2019 Apr 24;5(4):eaaw3754. doi: 10.1126/sciadv.aaw3754

Fig. 2. GH 32 family gene SpASFF1 from QTL AG3.2 shows trichome-specific expression.

Fig. 2

(A) Chromosome 3 with the AG3.2 QTL (30). Positions of three GH genes (Sopen03g040350, Sopen03g041640, and SpASFF1) are indicated. (B) Expression of GFP-GUS under control of the native ASFF1 promoter from S. pennellii LA0716 yields a GFP signal in S. lycopersicum M82 type I/IV trichome tip cells, but not in stalk cells or stem tissue. The green channel indicates the GFP signal; the magenta channel shows chlorophyll fluorescence. Scale bar, 100 μm. (C) qRT-PCR analysis of transcript abundance indicates that ASFF1 transcripts are higher in S. pennellii LA0716 trichomes than in underlying stem tissue but lower in S. lycopersicum M82 trichomes than in underlying stem tissue. Whiskers represent minimum and maximum values less than 1.5 times the interquartile range from the first and third quartiles, respectively. Values outside this range are represented as circles. *P < 0.05 and ***P < 0.001, Welch two-sample t test; n = 6 for all species and tissue types.