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. 2019 Apr 24;5(4):eaaw3754. doi: 10.1126/sciadv.aaw3754

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Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 5 — (A) LC-MS analysis of in vitro enzyme assay products indicates that SpASFF1 hydrolyzed P-type S3:19 (4^R4,5^R2,10^R3) acylsucrose, yielding two compounds with m/z 533.3. This m/z is consistent with an acylglucose product with a G3:19 (4,5,10) configuration; the two peaks represent the α and β anomers of the acylglucose. (B) LC-MS analysis of in vitro assays with F-type S3:22 (5^R4,5^R3′,12^R3) acylsucrose indicates no hydrolysis products with SpASFF1. Acylglucose structure is inferred from collision-induced dissociation-mediated fragmentation (fig. S9). All ESI− mode acylsugars were identified as formate adducts.