Table 2.

Level of protein abundance (biofilms versus early- and mid-stationary planktonic forms) obtained at different time points.

				Log2 fold change
Locus tag	Protein name	Protein description (COGs)	COG categories	Early biofilm	Maturing biofilm	Late biofilm	Early biofilm	Maturing biofilm	Late biofilm	References
				Versus early stationary phase			Versus mid stationary phase
Increased Abundance
BTH_II0679	trpB	tryptophan synthase, beta chain (EC 4.2.1.20)	E	1.69	1.47	1.50	1.29	1.78	1.57
BTH_I1550	glk	glucokinase (EC 2.7.1.2)/transcriptional regulator, RpiR family	G	1.16	1.40	1.66	1.91	1.74	1.99	(Kumar et al.2014)
BTH_I1553		carbohydrate ABC transporter substrate-binding protein	G	2.62	1.14	3.92	2.45	4.33	2.86	(Higgins 1992)
BTH_II1908	aepX	phosphoenolpyruvate mutase (EC 5.4.2.9)	G	1.26	1.52	1.25	1.52	1.31	1.57
BTH_I2608		3-oxoadipate CoA-succinyl transferase alpha subunit	I	1.88	2.06	2.56	2.74	2.48	2.66
BTH_I2245	purA	Adenylosuccinate synthetase (EC 6.3.4.4)	F	1.16	1.23	2.02	2.09	1.42	1.49
BTH_I0979	purT	formate-dependent phosphoribosylglycinamide formyltransferase	F	1.67	1.13	2.53	2.00	2.48	1.94
BTH_I3111	pyrE	orotate phosphoribosyltransferase (EC 2.4.2.10)	F	2.49	2.21	2.82	2.53	2.61	2.33
BTH_I0164	hslU	heat shock protein HslVU, ATPase subunit HslU	O	3.30	3.62	3.92	4.24	2.69	3.01
BTH_I2122	lon	ATP-dependent proteinase	O	1.35	1.58	1.48	1.71	1.90	2.13	(Rogers et al.2016)
BTH_I2092		antioxidant, AhpC/Tsa family	Q	1.39	2.13	2.51	3.25	2.03	2.77	(Chae et al.1994; Loprasert et al. 2003)
BTH_I2248	tex	transcription accessory protein, TEX	K	1.99	2.22	1.88	2.11	2.63	2.86	(He et al.2006; Moule et al.2015)
BTH_I3195	rpsU1	SSU ribosomal protein S21P	J	3.07	3.76	3.62	4.32	2.39	3.09
BTH_I3072	rpsG	SSU ribosomal protein S7P	J	1.66	2.02	1.71	2.07	1.43	1.79
BTH_I3064	rpsS	SSU ribosomal protein S19P	J	2.03	2.35	2.25	2.56	1.70	2.01
BTH_I3063	rplV	LSU ribosomal protein L22P	J	1.58	1.84	1.39	1.65	1.32	1.58
BTH_I3061	rplP	LSU ribosomal protein L16P	J	1.71	1.98	1.98	2.25	1.32	1.59
BTH_I3055	rpsN	SSU ribosomal protein S14P	J	2.72	2.20	3.66	3.14	3.65	3.13
BTH_I3052	rplR	LSU ribosomal protein L18P	J	1.64	1.71	1.75	1.82	1.38	1.44
BTH_I3047	infA	bacterial translation initiation factor 1 (bIF−1)	J	1.26	1.03	1.67	1.44	1.48	1.25
BTH_I3041	rplQ	LSU ribosomal protein L17P	J	2.72	2.69	3.09	3.06	2.55	2.52
BTH_I2592	rplT	LSU ribosomal protein L20P	J	1.51	1.81	1.04	1.33	1.26	1.56
BTH_I2181	rpsR	SSU ribosomal protein S18P	J	2.40	1.70	2.64	1.95	2.46	1.76
BTH_I1661	rpsP	SSU ribosomal protein S16P	J	1.70	1.95	1.97	2.22	1.98	2.23
Increased Abundance
BTH_I1234	rpsI	SSU ribosomal protein S9P	J	1.32	1.39	1.10	1.17	1.44	1.50
BTH_I1233	rplM	LSU ribosomal protein L13P	J	1.75	1.90	1.72	1.87	1.39	1.54
BTH_I0780	rpmB	LSU ribosomal protein L28P	J	2.39	2.39	2.49	2.49	2.68	2.68
BTH_I2965		hypothetical protein	S	3.71	4.29	2.43	3.01	3.35	3.93
Decreased Abundance
BTH_I3253	gcvP	glycine dehydrogenase (decarboxylating) alpha subunit	E	−2.07	−1.76	−1.72	−1.41	−2.76	−2.46
BTH_II0673	leuD	3-isopropylmalate dehydratase, small subunit (EC 4.2.1.33)	E	−1.62	−1.46	−3.06	−2.90	−2.68	−2.52
BTH_I3023	aroB	3-dehydroquinate synthase (EC 4.2.3.4)	E	−2.18	−1.71	−1.76	−1.29	−1.59	−1.11
BTH_I1617		glutamine amidotransferase, class I	E	−2.29	−3.44	−1.35	−2.50	−2.03	−3.18
BTH_I1050	leuA	2-isopropylmalate synthase (EC 2.3.3.13)	E	−2.95	−2.47	−1.96	−1.48	−2.18	−1.69
BTH_I0861		Orn/Lys/Arg decarboxylase	E	−1.58	−1.96	−1.97	−2.35	−1.29	−1.67
BTH_I0710	kynU	Kynureninase (EC 3.7.1.3)	E	−2.31	−2.88	−1.65	−2.22	−1.66	−2.23
BTH_I1894	eno	enolase (EC 4.2.1.11)	G	−1.22	−1.71	−1.57	−2.07	−1.12	−1.62	(Al-Maleki et al. 2015)
BTH_I0962	tal	transaldolase (EC 2.2.1.2)	G	−1.31	−1.47	−1.37	−1.52	−1.90	−2.06
BTH_II0631	gnd	6-phosphogluconate dehydrogenase (decarboxylating)	G	−2.49	−2.39	−1.84	−1.75	−2.24	−2.14
BTH_I0011	gspG	type II secretion system protein G (GspG)	N	−1.22	−1.99	−1.29	−2.05	−1.34	−2.11
BTH_I2082		penicillin-binding protein, 1A family	M	−2.02	−2.38	−2.17	−2.53	−1.71	−2.07
BTH_I2035	bamA	Beta-barrel assembly machine subunit BamA	M	−1.76	−1.76	−1.86	−1.86	−1.27	−1.27	(Anwari et al. 2012)
BTH_I0857		ompA family protein	M	−2.33	−2.44	−2.38	−2.48	−2.03	−2.14	(Smani et al.2014)
BTH_I0556	ubiB	2-octaprenylphenol hydroxylase (EC 1.14.13.-)	H	−2.00	−2.63	−2.01	−2.64	−1.86	−2.49
BTH_I3311	atpH	ATP synthase F1 subcomplex delta subunit	C	−1.44	−2.52	−1.55	−2.63	−1.66	−2.75
BTH_I1069	nuoI	NADH dehydrogenase subunit I (EC 1.6.5.3)	C	−1.93	−1.78	−2.26	−2.11	−1.91	−1.75
Decreased Abundance
BTH_I1068	nuoH	NADH dehydrogenase subunit H (EC 1.6.5.3)	C	−1.81	−1.85	−2.69	−2.73	−1.95	−1.99
BTH_I1064	nuoD	NADH dehydrogenase subunit D (EC 1.6.5.3)	C	−1.15	−1.63	−1.90	−2.39	−1.74	−2.23
BTH_I1063	nuoC	NADH dehydrogenase subunit C (EC 1.6.5.3)	C	−2.16	−2.33	−2.69	−2.86	−2.59	−2.76
BTH_II0658	mdh1	malate dehydrogenase (NAD) (EC 1.1.1.37)	C	−1.71	−2.48	−1.00	−1.77	−1.33	−2.09
BTH_I3101	paaK	phenylacetate-CoA oxygenase/reductase, PaaK subunit	C	−2.02	−1.82	−2.95	−2.75	−4.59	−4.40	(Grishin and Cygler 2015)
BTH_I2808	pckG	Phosphoenolpyruvate carboxykinase	C	−4.19	−3.69	−6.32	−5.83	−4.63	−4.14
BTH_I2554	lpdA	dihydrolipoamide dehydrogenase	C	−2.27	−2.25	−2.70	−2.67	−3.09	−3.07
BTH_I1654		electron transfer flavoprotein alpha subunit apoprotein	C	−1.30	−1.17	−2.00	−1.88	−2.18	−2.06
BTH_I1072		NADH dehydrogenase subunit L (EC 1.6.5.3)	C	−2.51	−2.85	−3.13	−3.47	−2.11	−2.45
BTH_I1067	nuoG	NADH dehydrogenase subunit G (EC 1.6.5.3)	C	−2.08	−1.64	−2.84	−2.40	−2.86	−2.42
BTH_I0608		cytochrome c family protein	C	−2.32	−2.68	−1.30	−1.66	−1.14	−1.50
BTH_II2187	acnD	aconitase (EC 4.2.1.3)	C	−4.15	−4.54	−6.23	−6.62	−4.64	−5.04
BTH_II2020		fumarase, class I, homodimeric (EC 4.2.1.2)	C	−1.85	−1.86	−3.41	−3.42	−3.78	−3.79
BTH_II0663		succinate dehydrogenase subunit B (EC 1.3.5.1)	C	−1.31	−1.38	−2.11	−2.18	−1.99	−2.05
BTH_II0662	sdhA	succinate dehydrogenase subunit A (EC 1.3.5.1)	C	−2.17	−2.28	−3.38	−3.49	−2.24	−2.35
BTH_II0654	acnA	aconitase (EC 4.2.1.3)	C	−1.82	−1.95	−2.31	−2.45	−1.94	−2.07
BTH_II0362		succinate dehydrogenase subunit B (EC 1.3.5.1)	C	−3.19	−3.76	−3.86	−4.44	−2.88	−3.46
BTH_I2916		PDZ domain protein	R	−1.97	−1.37	−1.88	−1.28	−1.59	−1.00
BTH_I1673		microcin-processing peptidase 1	R	−3.91	−3.49	−5.64	−5.21	−4.54	−4.12
BTH_I1296		microcin-processing peptidase 2	R	−4.03	−3.18	−2.69	−1.84	−2.50	−1.65
BTH_I2765		exopolyphosphatase	P	−1.53	−2.16	−3.14	−3.77	−2.89	−3.51	(Kornberg, Rao and Ault-Riche 1999; Malde et al. 2014; Chuang et al. 2015)
BTH_I1276	secD	protein-export membrane protein SecD	U	−1.18	−1.09	−1.48	−1.38	−1.46	−1.36	(Sachelaru et al. 2017)
BTH_I2255		poly(R)-hydroxyalkanoic acid synthase, class I	I	−3.22	−2.30	−3.58	−2.67	−2.36	−1.45
BTH_I0973	phaZ	intracellular PHB depolymerase	I	−3.47	−3.54	−2.76	−2.84	−2.30	−2.37
Decreased Abundance
BTH_I1250	purH	bifunctional purine biosynthesis protein PurH	F	−2.17	−3.13	−1.70	−2.66	−1.74	−2.70
BTH_I0739	adk	Adenylate kinase (EC 2.7.4.3)	F	−2.97	−3.57	−2.81	−3.40	−2.31	−2.91	(Markaryan et al.2001)
BTH_I2048		SPFH domain, Band 7 family protein	O	−2.79	−2.88	−1.95	−2.04	−1.67	−1.76
BTH_I0415		carboxy-terminal protease	O	−2.05	−2.51	−1.22	−1.68	−1.78	−2.24	(Bandara et al. 2008)
BTH_I2088	mfd	transcription-repair coupling factor	L	−1.46	−1.38	−1.46	−1.37	−1.48	−1.40	(Roberts and Park 2004)
BTH_I1388		1-Cys peroxiredoxin (EC 1.11.1.15)	Q	−2.60	−3.32	−2.31	−3.03	−1.54	−2.27	(Kaihami et al. 2014)
BTH_I0756	fusA1	translation elongation factor 2 (EF-2/EF-G)	J	−1.32	−1.76	−1.59	−2.03	−1.12	−1.56
BTH_I0485	raiA	SSU ribosomal protein S30P/sigma 54 modulation protein	J	−2.06	−2.19	−2.13	−2.26	−2.09	−2.22
BTH_I2218		hypothetical protein	S	−1.99	−2.20	−1.74	−1.95	−1.61	−1.82
BTH_I1867		hypothetical protein	S	−3.96	−4.14	−1.47	−1.66	−2.18	−2.37
BTH_I1491		hypothetical protein	S	−5.27	−5.53	−5.38	−5.64	−4.64	−4.90
BTH_I1191		hypothetical protein	S	−2.91	−2.73	−2.53	−2.36	−2.33	−2.15
BTH_I1155		hypothetical protein	S	−2.14	−2.06	−1.70	−1.62	−1.79	−1.71
BTH_I0605		hypothetical protein	S	−1.75	−1.95	−1.88	−2.08	−1.67	−1.86
BTH_I0454	cydA	cytochrome d ubiquinol oxidase subunit I	C	−3.90	−4.28	−5.61	−5.98	−4.19	−4.56