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. Author manuscript; available in PMC: 2020 Apr 23.
Published in final edited form as: ACS Nano. 2019 Apr 3;13(4):4469–4477. doi: 10.1021/acsnano.9b00008

Figure 5: Single-molecule kinetics of the Syn B2-αHL interactions.

Figure 5:

(A) The dependence of the kinetic rate constant of association, kon, on the concentration of less-penetrating PEGs; (B) The dependence of the kinetic rate constant of dissociation, koff, on the concentration of less-penetrating PEGs; (C) Free energy, ΔG, of the Syn B2-αHL complex formation in the presence of PEG-2k (black squares), PEG-4k (red circles) and PEG-8k (blue triangles). PEGs were symmetrically added to both sides of the chamber. The other experimental conditions were the same as those stated in the caption of Fig. 2.