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. Author manuscript; available in PMC: 2020 Apr 22.
Published in final edited form as: Curr Biol. 2019 Apr 4;29(8):1286–1300.e4. doi: 10.1016/j.cub.2019.02.062

Figure 6. Destabilizing axonemal MTs or disrupting tubulin-IFT interactions suppresses the dyf-18 AWA cilia phenotype.

Figure 6.

A, C) Representative images of AWA cilia morphologies in animals of the indicated genotypes. AWA neurons were visualized via expression of gpa-4Δ6p::myr-gfp. The cilium base and cilium are indicated by yellow and white arrowheads, respectively; the dendrite is marked by an arrow. Alleles used in C were dyf-18(ok200), ift-81(tm2355), ift-74(tm2393). Anterior is at top or at top left in all images. Scale bar: 10 μm. Animals were grown at 15°C in A.

B, D) Percentage of AWA neurons in adult hermaphrodites of the indicated genotypes exhibiting cilia phenotypes. Alleles used in D were dyf-18(ok200), ift-81(tm2355), ift-74(tm2393). Numbers in each bar indicate the number of examined neurons in 2-3 independent experiments. *** and ### indicate different from wild-type and indicated values, respectively, at P<0.001; ns – not significant (Wilcoxon rank-sum test). Animals were grown at 15°C in B.

Also see Figure S3, Figure S4, and Table S2.