Table 2.
Spheroid, astrocyte, microglia and phagocyte evaluation in rNCC
| Parenchyma | P‐value* | Meninges | P‐value† | Ventricle | ||||
|---|---|---|---|---|---|---|---|---|
| Intracranial route | Oral route | Intracranial route | Oral route | Intracranial route | ||||
| Glial cells | GFAP | 25.60 ± 5.67 | 12.77 ± 4.20 | 0.24 | 15.68 ± 3.12 | 34.32 ± 10.45 | 0.06 | 12.62 ± 4.33 |
| Iba‐1 | 5.24 ± 1.57 | 5.33 ± 2.04 | 0.58 | 2.20 ± 0.55 | 0.92 ± 0.53 | 0.34 | 3.74 ± 1.13 | |
| CD68 | 2.98 ± 0.58 | 2.13 ± 1.65 | 0.48 | 1.10 ± 0.50 | 0.41 ± 0.23 | 0.63 | 2.65 ± 1.68 | |
| Frequency (%) of spheroids | p‐NFP200kD | 64% | 100% | 0.51 | 88% | 75% | 0.51 | 80% |
| SOD1 | 82% | 100% | 1.00 | 94% | 100% | 1.00 | 40% | |
| Ubiquitin | 72% | 75% | 1.00 | 100% | 100% | – | 60% | |
| Mean no. of spheroids per cysticercus | p‐NFP200kD | 110.63 ± 23.90 | 45.50 ± 25.60 | 0.10 | 35.30 ± 10.70 | 38.00 ± 21.00 | 0.96 | 32.00 ± 14.00 |
| np‐NFP200kD | 16.60 ± 10.30 | 33.30 ± 20.53 | 0.29 | 8.00 ± 3.99 | 3.00 ± 0.00 | 0.58 | – | |
| pan NFP200kD | 44.50 ± 17.67 | 66.60 ± 39.89 | 0.72 | 14.00 ± 7.46 | 12.30 ± 7.50 | 0.84 | 2.00 ± 2.00 | |
| SOD1 | 150.00 ± 22.40 | 144.00 ± 67.10 | 0.58 | 64.40 ± 28.30 | 69.70 ± 18.80 | 0.39 | 92.40 ± 40.60 | |
| Ubiquitin | 97.10 ± 19.90 | 129.00 ± 64.90 | 0.07 | 53.90 ± 15.80 | 39.00 ± 18.20 | 0.82 | 53.70 ± 32.30 | |
| Hsp70 | 36.00 ± 23.40 | 22.00± | 0.76 | 20.00 ± 9.96 | 6.66 ± 4.05 | 0.84 | – | |
Neuronal lesion evaluation in rNCC. For glial cells and phagocytes, the histological area analyzed was up to 300 µm from the collagen layer and is presented as the mean area of immunoreactivity ± SEM. For the frequency (%) of spheroids and spheroid number, the histological area analyzed was up to 1500 µm from the collagen layer. The frequency (%) of spheroids shows the proportion of cysticerci that are associated with any number of spheroids; statistics performed using Fisher exact test. Spheroid number is the mean number of spheroids per cysticerci and is presented as mean ± SEM. Sham groups from either intracranial or oral routes of infection did not show any structure resembling spheroids. For np‐NFP200kD we only performed IHC for 3 ventricular, 6 parenchymal and 13 meningeal cysticerci, and for Hsp70 only 1 ventricular, 3 parenchymal and 9 meningeal cysticerci were analyzed.
P‐value represents individual P‐values of the two‐sample test of ranges (Mann‐Whitney) between each variable for parenchymal cysticerci comparing intracranial vs. oral route.
P‐value represents individual P‐values of the two‐sample test of ranges (Mann‐Whitney) between each variable for meningeal cysticerci comparing intracranial vs. oral route.