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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: Eur J Pharm Sci. 2019 Mar 28;133:86–94. doi: 10.1016/j.ejps.2019.03.020

Fig. 6. p97 interacts with NP and NMS-873 retains NP in the nucleus.

Fig. 6.

(A) Co-IP of Flag-p97 and NP-HA. HEK 293 cells were transfected with Flag-p97 alone or along with NP-HA. After 24 h post transfection, cell lysates were subjected to IP using anti-Flag antibody (IP Flag), followed by Western blotting using anti-HA (IB HA) or anti-His (IB His) anti-bodies, respectively. (B) Co-IP of p97 and NP. A549 cells infected with influenza A/WSN/33 (H1N1) at MOI of 3 were used for co-immunoprecipitation assay. Cell lysates were subjected to IP using anti-p97 antibody (IP p97), followed by Western blotting using anti-p97 (IB p97) or anti-NP (IB NP) antibodies, respectively. (C) NMS-873 retains NP in the nuclear. A549 cells were transfected with Flag-p97 alone or along with NP-HA. At 2 h post transfection, compound was added into the culture medium. At 24 h post transfection, cells were fixed and stained with mouse anti-Flag antibody, rabbit anti-HA and DAPI to determine the Flag-p97, NP-HA, and nucleus, respectively.