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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Burns. 2019 Mar 2;45(3):627–640. doi: 10.1016/j.burns.2018.08.019

Figure 4: Neutrophils from burn mice are not able to be activated after secondary infection to increase RONS production.

Figure 4:

Mice underwent sham or burn injury and were infected (17 days after injury) either IP with P. aeruginosa (ShamP or BurnP) or IT with P. aeruginosa 14 days after injury followed by IP infection at 17 days after injury (ShamT/P or BurnT/P) or left uninfected (Sham or Burn). Lungs were harvested 24 hours later; (A, B, C,D) reactive oxygen / nitrogen oxidative (RONS) ability per cell was measured by flow cytometry in the absence or presence of Phorbol myristate acetate (PMA) stimulation (“+Stim.”), E) NOS2 expression per cell was quantified in neutrophils using flow cytometry. Data shown are *p<0.05, **p<0.01, with geometric mean highlighted and are representative of three repeated experiments.