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Quantification of birefringence measured in aortic valve leaflets stained with picrosirius red showing birefringence as a measure of total area within the fibrosa (A) and spongiosa (B). Color of birefringence as a percentage of total birefringence in the fibrosa (C) and spongiosa (D). Red refers to red birefringence (bottom), followed by orange, yellow and green.
