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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Mar 5;1864(7):1061–1071. doi: 10.1016/j.bbalip.2019.03.001

Figure 8.

Figure 8.

Secondary structure and thermal stability of VLDL and LDL from healthy and diabetic subjects. Lipoproteins (batch 1, Table S1) were isolated from pooled plasma of normolipidemic subjects (NL, black) and of diabetic patients with either good glycemic control, A1c<7% (GC, light gray), or poor glycemic control, A1c≥7% (PC, dark gray). The lipoproteins were obtained as described in part 2.2 and the data were recorded as described in part 2.5 Methods. For VLDL (top panels), far-UV CD spectra (A) and the CD melting data at 220 nm (B) are shown. The melting data monitor changes in the protein secondary structure upon heating and cooling. For LDL (bottom panels), far-UV CD spectra (C) and the melting data recorded by turbidity at 320 nm (D) are shown. The turbidity data monitor changes in the particle size during thermal remodeling. Arrows in panels B and D show directions of the temperature changes.