Skip to main content
. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Mar 22;1864(7):985–1004. doi: 10.1016/j.bbalip.2019.03.009

Figure 10. Pair-fed high glucose diet (HGD) has greater effect than Lfabp gene ablation (LKO) on protein levels of key liver fatty acid transporters.

Figure 10.

All conditions were as in legend of Fig. 9 except that SDS-PAGE and Western blotting were performed to determine relative hepatic protein levels of: Group 1: membrane fatty acid transport proteins, i.e. FATP2 (Fig. 10A; Supplemental Fig. 7B), FATP4 (Fig. 10B; Supplemental Fig. 11A), and FATP5 (Fig. 10C; Supplemental Fig. 11B) and GOT (Fig. 10D; Supplemental Fig. 12A); Group 2: cytosolic fatty acid/fatty acyl CoA binding proteins, i.e. FABP1 (Fig. 10E; Supplemental Fig. 4A, 9B, 12A), SCP2 (Fig. 10F; Supplemental Fig. 6B, 8B,13A), 3-αHSD (Fig. 10G; Supplemental Fig. 10A), ACBP (Fig. 10H; Supplemental Fig. 8A, 11B), and FABP2 (Fig. 10I; Supplemental Fig. 10B). Western blot images for each protein (n=7) along with GAPDH, COX4, or β-actin housekeeper for normalization are shown in the respective Supplemental Figs. 4–17. WT (Black bars) and LKO (Open bars). Values represent the mean ± SEM, n=7, with statistical significance indicated as follows: * p≤ 0.05 vs WT mice on control diet; @ p≤ 0.05 vs LKO mice on control diet; and #p≤ 0.05 vs WT mice HGD.