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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Mar 22;1864(7):985–1004. doi: 10.1016/j.bbalip.2019.03.009

Figure 8. Impact of Lfabp gene ablation (LKO) and pair-fed high glucose diet (HGD) on serum VLDL, LDL, and HDL protein content and lipid composition.

Figure 8.

All conditions were as described in legends of Fig. 1,2. At the end of the dietary study, fasting serum was collected from wild-type (WT) and LKO mice for lipoprotein profiling as in Fig. 8 and individual lipoprotein fraction (VLDL, LDL, HDL) isolation as described in Methods. One aliquot of each VLDL, LDL, and HDL fraction was used for determination of protein content (A) as described in Methods while the remainder was used for lipid extraction and individual class distribution also as described in Methods: B. % Total lipid; C. % Triacylglycerol; D. % Total Cholesterol; E. % Free Cholesterol; F. % Cholesterol Ester; G, % Phospholipid; H. C/PL ratio (Free Cholesterol)/Phospholipid ratio. In each panel the bars in each group refer from left to right indicate WT control diet (black bar); LKO control diet (open bar); WT HGD (dark shaded bar); LKO HGD (light shaded bar). Values represent the mean ± SEM (n=6–8). Statistical analysis was performed within each lipoprotein class as described in Methods as follows: * p≤ 0.05 vs WT mice on control diet; @ p≤ 0.05 vs LKO mice on control diet; and #p≤ 0.05 vs WT mice on HGD.