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. 2019 Apr 24;10(5):343. doi: 10.1038/s41419-019-1577-2

Fig. 4. O-GlcNAcylation is involved in the control of GFAT transcription in Nic-treated breast cancer cells.

Fig. 4

a MCF-7 and MDA-MB-231 cells were treated with 100 μM Nic either alone or together with L01 (100 μM)/PugNAc (50 μM) for 24 h. The lysates were subjected to ChIP assays. Quantitative PCR amplification was performed using primers specific for GFAT promoter. b Indicated cells were transfected with a reporter vector consisting of luciferase cDNA fused to GFAT promotor. Transfection of OGT siRNA or inhibition of O-GlcNAc (100 μM L01) decreased GFAT promotor activity. PugNAc (50 μM) increased GFAT promotor activity. c O-GlcNAcylation regulates GFAT transcription in breast cancer cells with Nic treatment (100 μM, 24 h). Transfection of OGT siRNA or inhibition of O-GlcNAc (100 μM L01) decreased GFAT transcription. PugNAc (50 μM) increased GFAT transcription. GFAT transcript level was analyzed by quantitative RT-PCR. Scrambled siRNA was used as a control (CTRL). The data represent the mean ± SEM, N = 3, *p < 0.05, **p < 0.01