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. 2019 Apr 24;9:6497. doi: 10.1038/s41598-019-42880-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental timeline and sample sizes for testing how time of day modulates behavioral and neuroinflammatory changes in tumor-free (surgical controls), tumor-bearing, and tumor-resected mice. Tumor-resected mice were surgically inoculated with mammary tumor cells. Between 2–3 weeks later, tumor-resected mice had tumors surgically removed, tumor-free control mice received a tumor resection sham surgery and tumor-bearing mice received a tumor induction. Behavior was tested during light or dark phase 2–3 weeks later, followed by tissue collection. The syringes and scalpel drawings have been obtained from free clipart through Microsoft PowerPoint, released under CC-BY-SA 4.0 license (the syringe is available at https://thumb9.shutterstock.com/photos/thumb_large/349072/142860406.jpg and the scalpel by Petit B https://creativecommons.org/licenses/by-sa/4.0, from Wikimedia Commons).