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. 2019 Apr 24;9:6480. doi: 10.1038/s41598-019-42795-6

Table 1.

Illumina libraries constructed. Two genomic DNA libraries (300, 550 bp) constructed from each of the source materials. For RNA sequencing, one library for each source was constructed.

DNA
Sample type Library type Average insert size [bp] No. of reads raw data [bp] %Q30b Mean Qa No. of reads after trimming & reduction [bp] Final depth [X]
Male (Haploid) Paired-end 125b × 2 290 84,756,564 90.34 34.28 74,249,558 84.4
Male (Haploid) Paired-end 125b × 2 510 95,513,084 88.63 33.9 82,214,665 93.4
Worker (Diploid) Paired-end 125b × 2 340 120,165,717 93.15 35.21 74,661,870 84.8
Worker (Diploid) Paired-end 125b × 2 560 105,253,786 90.44 34.56 83,032,369 94.4
RNA
Male Paired-end 125b × 2 210 184,860,252 93.07 35.13 173,306,486 N/A
Pool Paired-end 125b × 2 240 173,787,824 93.5 35.22 173,787,824 N/A

aMean Phred Q scores for each of the libraries, b%Q30 is the percentage of bases with a quality score of at least 30 for each library.