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. 2019 Apr 24;9:6490. doi: 10.1038/s41598-019-43021-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Enhanced induction of keratinocyte-like state by combinations of TP63/KLF4 and OVOL2. (A) Changes in marker gene expression upon inducible expression of TK and OVOL2. Keratinocyte lineage markers KRT14 and TP63, an E marker CDH1, and an M marker VIM were quantified by RT-qPCR after DOX induction of the indicated factors in HNDF. (B) Induction of KRT14 + population upon inducible expression of TK and OVOL2. A keratinocyte marker KRT14-positive population was assessed by flow cytometry after DOX induction of the indicated factors in HNDF.