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. 2019 Apr 18;10:381. doi: 10.3389/fneur.2019.00381

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Copyright © 2019 Zhao, Zheng, Yang, Fan, Zhu, Liu, Wu and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of CTs on apoptosis-related proteins expressions in neurons in OGD/R-injured NVU model in vitro. (A) Representative Western blots showing the levels of Caspase-3, cleaved-Caspase-3(17, 19 kD), PARP and cleaved-PARP in neurons. (B) Relative density refers to the ratio of cleaved-Caspase-3(17, 19 kD) to Caspase-3 and cleaved-PARP to PARP (n = 4). (C) Representative Western blots showing the levels of Bax and Bcl-2 in neurons. (D) Relative density refers to the ratio of Bax to Bcl-2 (n = 4). The semiquantitative analyse results indicated that CTs protected neurons from apoptosis induced by OGD/R. Data are presented as the mean±SD. ^**P < 0.01 vs. Control group; ^#P < 0.05, ^##P < 0.01 vs. OGD2h/R24h group. PARP, poly-ADP-ribose polymerase; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; CTs, cryptotanshinone.