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. 2019 Apr 24;9:6520. doi: 10.1038/s41598-019-43016-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcriptional profiles of ECOH cells treated with or without (a) eugenol-NE and (b) methyl salicylate-NE for 24 h and expression of targeted genes was quantified by qRT-PCR. Relative gene expressions represent transcriptional levels after exposure to eugenol-NE and methyl salicylate-NE versus untreated controls (value 1.0). The mixture of DW, surfactant (S; Tween 80) and co-surfactant (CS; propylene glycol) in the ration of 94.7:0.2:0.1 was used as a solvent control. The data were expressed as arithmetic mean ± SE. The experiments were carried out at least thrice in independent experiments having three replicates. *P < 0.05 versus non-treated controls.