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. 2019 Apr 24;9:6475. doi: 10.1038/s41598-019-42931-2

Figure 7.

Figure 7

Aβ inhibits F0F1 ATP synthase activity in isolated and permeabilized mitochondria. (a) mitochondria isolated from wt N13 or N13R cells; (b) liver mitochondria isolated from P2X7R wt or P2X7R-KO mice (see Methods). Mitochondria were suspended at a concentration of 5–10 mg/mL, permeabilized by two rounds of freeze/thawing, exposed to Aβ or iAβ at a concentration of 2 μM, and assayed for ATP synthase activity as described in Methods. Data are means + SEM from 3 independent experiments, each performed in triplicate for a total of 9 individual determinations. **p < 0.01; ***p < 0.001; ****p < 0.000.1.