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. 2019 Apr 24;10:1894. doi: 10.1038/s41467-019-09856-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Dynamic and precise ion-pairing (rendezvous) of an siRNA with free YBCs. a Schematic illustration of the FRET-based analysis of dynamic ion-pairing of siRNA with free YBC (or the exchange reaction between uPIC-forming YBC and free YBC). b FRET analysis of uPIC formation and exchange reaction in buffer solution. c–e IVCLSM images of blood vessels in the mouse ear dermis treated with a stepwise systemic injection of A594-YBC (c), A647-siRNA (d), and N-YBC (e). The scale bars indicate 50 µm. f Time-dependent changes in the FRET signal obtained by quantitative analysis of the IVCLSM images acquired after A594-YBC injection. Left and right arrows indicate the injection of A647-siRNA and non-labelled YBC (N-YBC), respectively. g Sample preparation scheme for the comparative study of uPIC formation under varying conditions. h Hydrodynamic diameters of A647-siRNA complexed with YBC under varying conditions. Data represent the means ± s.d. for five replicate measurements