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. 2019 Apr 24;9:6496. doi: 10.1038/s41598-019-42653-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of HsbA variant on biofilm formation and swimming motility. (A) Quantification of biofilm production after Crystal Violet-staining. Corresponding levels of biofilm production represent mean values and standard deviations obtained from three independent experiments. (B) Swimming mobility on soft agar plate was illustrated (upper panel) and colony size surface was measured (lower panel). Colony surface is expressed in square cm and correspond to mean values and standard deviations obtained from three independent experiments. Wilcoxon-Mann-Whitney tests were performed and *, **, *** and ns referred to p < 0.05, p < 0.01 and p < 0.001 and nonsignificant difference, respectively.