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. 2019 Apr 24;10(5):342. doi: 10.1038/s41419-019-1568-3

Fig. 4. Combinations of BH3-mimetics induce apoptosis in melanoma cells as determined by Annexin V / propidium iodide staining.

Fig. 4

Co-targeting of MCL-1 plus BCL-XL is more effective than the co-targeting of MCL-1 plus BCL-2, consistent with the CellTiter-Glo assay data. For most cell lines, potent melanoma cell killing was observed after 24 h treatment when S63845 was combined with either ABT-263 or A1331852 at a concentration where both drugs had no major effect on their own (i.e., 1 μM). Melanoma cell killing was inhibited by the addition of the pan-caspase inhibitor Q-VD-OPh, consistent with induction of apoptosis. Data represent mean ± standard deviation from N = 3 separate assays