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. 2019 Apr 24;10(5):342. doi: 10.1038/s41419-019-1568-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a A02, b A375, c LM-MEL-28 cells were allowed to form spheroids over 72 h and then treated for 24 h with drugs or combinations as indicated. Bright field images (left panel) and fluorescence images for DRAQ7 staining (right panel) are shown for each combination. The panel in the top right (red box) represents the vehicle only control. Spheroids were imaged with an IN Cell Analyzer 2200/Nikon ×4/0.20, Plan Apo, CFI/60. Note differences in DRAQ7 intensity and distribution. Data is representative from N = 3 independent experiments