Figure 1.

SAX-HPLC chromatogram of Heparin batch Man_D-2. Some disaccharides show a double peak, identified by the extension “-i,” depending on the elution of the two anomeric forms of the reducing end terminal. Early peaks, eluting in the range between 0 and 5 min, are system peaks. Other very small peaks could not be assigned, but no sample has shown an un-identified peak area larger than 0.25%, or a total area of un-identified peaks larger than 1.2%.