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. 2019 Apr 18;6:78. doi: 10.3389/fmed.2019.00078

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Copyright © 2019 Spelta, Liverani, Peluso, Marinozzi, Urso, Guerrini and Naggi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of results for the content of glucosamine with a sulfate in position 6 (GlcNx,6S): results from SAX-HPLC vs. HSQC (blue dots) and ¹³C-NMR (red squares). The distribution of blue dots shows a clear correlation between the content of 6-sulfated glucosamine from the two methods, where results from the SAX method are always higher than those from the HSQC. Quantitative results from the carbon spectra of a sub-set of 6 heparin samples (red squares) show a much better agreement with SAX results, confirming a greater accuracy of the enzymatic method than the HSQC.