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. 2019 Apr 18;10:639. doi: 10.3389/fmicb.2019.00639

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Copyright © 2019 Kroupitski, Gollop, Belausov, Pinto and Sela (Saldinger).

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of growth medium on the internalization of Salmonella in lettuce leaf. Salmonella was grown overnight in the following media: LB, LBNS, water-diluted LB (1:1) with shaking at 37°C, washed and tested for leaf internalization. Incidence of bacteria in internal leaf tissues was calculated as the percentage of microscopic fields (×40) harboring ≥1 mCherry labeled bacteria in 30 randomly chosen microscopic fields of leaf tissue. The data denote the average and standard error (SE) of four independent experiments each performed in triplicates (3 × 30 fields per experiment). Different letters indicate significant difference (P < 0.05) between means of internal fields harboring bacteria, according to ANOVA Tukey–Kramer Multiple Comparisons Test.